Salk scientists and collaborators have shed light on a long-standing question about what leads to variation in stem cells by comparing induced pluripotent stem cells (iPSCs) derived from identical twins. Even iPSCs made from the cells of twins, they found, have important differences, suggesting that not all variation between iPSC lines is rooted in genetics, since the twins have identical genes. Because they can differentiate into almost any cell type in the body, stem cells have the potential to be used to create healthy cells to treat a number of diseases. But stem cells come in two varieties: embryonic stem cells (ESCs), which are isolated from embryos, and iPSCs, which are created in the lab from adult cells that are reprogrammed using mixtures of signaling molecules and are a promising tool for understanding disease and developing new treatments. Although iPSCs resemble ESCs in most ways, scientists have found that iPSCs often have variations in their epigenetics -- methyl marks on the DNA that dictate when genes are expressed. These epigenetic markers aren't the same between iPSCs and ESCs, or even between different lines of iPSCs. In the past, it's been hard to determine what drives these differences. "When we reprogram … Continue reading
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