Medicine in the post-genomic era Genome Medicine publishes peer-reviewed research articles, new methods, software tools, reviews and comment articles in all areas of medicine studied from a post-genomic perspective. Areas covered include, but are not limited to, disease genomics (including genome-wide association studies and sequencing-based studies), disease epigenomics, pathogen and microbiome genomics, immunogenomics, translational genomics, pharmacogenomics and personalized medicine, proteomics and metabolomics in medicine, systems medicine, and ethical, legal and social issues. There has been an error retrieving the data. Please try again. DNA-PK inhibition boosts Cas9-mediated HDR Transient pharmacological inhibition of DNA-PKcs can stimulate homology-directed repair following Cas9-mediated induction of a double strand break, and is expected to reduce the downstream workload. Genomics of epilepsy Candace Myers and Heather Mefford review how advances in genomic technologies have aided variant discovery, leading to a rapid increase in our understanding of epilepsy genetics. CpG sites associated with atopy Thirteen novel epigenetic loci associated with atopy and high IgE were found that could serve 55 as candidate loci; of these, four were within genes with known roles in the immune response. Longitudinal 'omic profiles A pilot study quantifying gene expression and methylation profile consistency over a year shows high longitudinal consistency, with … Continue reading →

Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.[1][2] The 19th-century English physiologist Sydney Ringer developed salt solutions containing the chlorides of sodium, potassium, calcium and magnesium suitable for maintaining the beating of an isolated animal heart outside of the body.[3] In 1885, Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture.[4]Ross Granville Harrison, working at Johns Hopkins … Continue reading →

Gillette Childrens Specialty Healthcare is a regional leaderin pediatric neurology and neurosurgery. Our Center for Pediatric Neurosciences offers expert diagnoses and treatments for seizures and epilepsy in children and teens. Our goal is to work closely with families to provide the most effective management and best care for children who have epilepsy. Many of our patients who have epilepsy also have complex associated conditions, such as cerebral palsy, developmental delays or traumatic brain injuries. Thats why we offer a large team of specialists and extensive support services. Our focus is on caring for the whole child. Our brains have constant electrical activity in and between cells. A seizure is an abnormal electrical discharge in brain cells that causes a change in normal activity, behavior or movement. Epilepsy is diagnosed when a person has two or more unprovoked seizures. A person who has only provoked seizures (febrile seizures, seizures after head trauma) will not receive an epilepsy diagnosis. Epilepsy can have many causes. The neurologist will order tests to help determine why a child has epilepsy. Some of the known causes for epilepsy include: Seizures range from mild to severe. Indications that a person is having a seizure include: We use … Continue reading →

Stem cell facts Stem cells are cells that have the potential to develop into many different or specialized cell types. Stem cells can be thought of as primitive, "unspecialized" cells that are able to divide and become specialized cells of the body such as liver cells, muscle cells, blood cells, and other cells with specific functions. Stem cells are referred to as "undifferentiated" cells because they have not yet committed to a developmental path that will form a specific tissue or organ. The process of changing into a specific cell type is known as differentiation. In some areas of the body, stem cells divide regularly to renew and repair the existing tissue. The bone marrow and gastrointestinal tract are examples of areas in which stem cells function to renew and repair tissue. The best and most readily understood example of a stem cell in humans is that of the fertilized egg, or zygote. A zygote is a single cell that is formed by the union of a sperm and ovum. The sperm and the ovum each carry half of the genetic material required to form a new individual. Once that single cell or zygote starts dividing, it is known as … Continue reading →