REDWOOD CITY, CA--(Marketwired - Mar 21, 2017) - Synthego, a leading provider of genome engineering solutions, today announces the availability of chemically modified synthetic guide RNA in the CRISPRevolution product family, including modified full length 100-mer synthetic single guide RNA (sgRNA). Critical for editing challenging cell types such as primary and stem cells, modified guide RNAs provide protection against intracellular immune responses present in primary cells, such as stem cells. Additionally, chemically modified RNA improves in vivo stability by protecting RNA molecules against exonuclease attack -- allowing it to persist longer in certain cell types and regions of genomic DNA that can be challenging to edit using conventional guide RNAs. Synthego's modified sgRNA are synthesized with 2'-O-methyl analogs and 3' phosphorothioate internucleotide linkages in the first three nucleotides at both the 5' and 3' end of the RNA molecule. It's currently impossible to make chemical modifications to plasmid or in vitro transcription (IVT) based guide RNAs. Synthetic sgRNA is chemically synthesized to the highest levels of purity, eliminating the risk of introducing toxins that may be present in IVT-based guide RNAs. In addition, the use of modified synthetic sgRNA also safeguards against the accidental incorporation of DNA into edited stem … Continue reading
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