16 hours ago A 3-D visualization of fluorescently-labeled brain cells within an intact brain tissue. Through the use of this novel whole-body clearing and staining method, researchers can make an organisms tissues transparentallowing them to look through the tissues of an organism for specific cells that have been labeled or stained.Credit: Bin Yang and Viviana Gradinaru/Caltech (Phys.org) In general, our knowledge of biologyand much of science in generalis limited by our ability to actually see things. Researchers who study developmental problems and disease, in particular, are often limited by their inability to look inside an organism to figure out exactly what went wrong and when. Now, thanks to techniques developed at Caltech, scientists can see through tissues, organs, and even an entire body. The techniques offer new insight into the cell-by-cell makeup of organismsand the promise of novel diagnostic medical applications. "Large volumes of tissue are not optically transparentyou can't see through them," says Viviana Gradinaru, an assistant professor of biology at Caltech and the principal investigator whose team has developed the new techniques, which are explained in a paper appearing in the journal Cell. Lipids throughout cells provide structural support, but they also prevent light from passing through the … Continue reading
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